Assembly errors cause false tandem duplicate regions in the chicken (Gallus gallus) genome sequence.
Chromosoma
; 123(1-2): 165-8, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24213641
ABSTRACT
The complexity of eukaryote genomes makes assembly errors inevitable in the process of constructing reference genomes. Next-generation sequencing (NGS) could provide an efficient way to validate previously assembled genomes. Here, we exploited NGS data to interrogate the chicken reference genome and identified 35 pairs of nearly identical regions with >99.5 % sequence similarity and a median size of 109 kb. Several lines of evidence, including read depth, the composition of junction sequences, and sequence similarity, suggest that these regions present genome assembly errors and should be excluded from forthcoming genomic studies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Galinhas
/
Genoma
/
Duplicação Gênica
/
Sequências de Repetição em Tandem
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article