[Development of full-quantified HPLC fingerprint for quality evaluation of ophiopogonis radix of sichuan].

ABSTRACT

OBJECTIVE: To establish HPLC fingerprint of Ophiopogonis Radix of Sichuan and simultaneously determine two homoisoflavonoids (methylophiopogonanones A and B). METHODS: Full-quantified HPLC fingerprint was used to establish the HPLC fingerprint and determine the active ingredients of the daodi medicinal material Ophiopogonis Radix of Sichuan in Shengmai injection. Chromatographic condition was as follows The analytical column was Waters symmetry shield RP 18 (4.6 mm x 250 mm, 5 microm) with a pre-column of symmetry shield RP 18. The mobile phase was acetonitrile-water (containing 0.1% phosphoric acid) with gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was set at 280 nm and the column temperature was maintained at 30 degrees C. RESULTS: The HPLC fingerprint of Ophiopogonis Radix of Sichuan was established with good separation and repeatability. 24 common peaks were defined in the HPLC fingerprint. The similarity among batches was more than 0.98. Compared with standard reference substances, No. 14 peak was methylophiopogonanone A and No. 15 peak was methylophiopogonanone B. Similarity determine system was applied to evaluate them. CONCLUSION: This analytical method is highly sensitive with strong specificity, which can be used efficiently in the quality control of Ophiopogonis Radix of Sichuan in Shengmai injection.