[Spectrum-effect relationship between anti-HIV 1 activities and ultra-performance liquid chromatography fingerprints of Rheum species].
Zhongguo Zhong Yao Za Zhi
; 38(15): 2434-7, 2013 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-24228530
ABSTRACT
OBJECTIVE:
To study the relationship between ultra-performance liquid chromatography (UPLC) fingerprints of Rheum species and their anti-HIV 1 activities.METHOD:
Twenty two samples of 16 species belonging to genus Rheum from various sources were collected and analyzed in this study. Firstly they were assayed for the HIV-1 reverse transcriptase (RT) associated ribonuclease H (RNase H) activity. Secondly the fingerprints were established by an optimized UPLC method. Sample was analyzed by UPLC-TOF-MS/MS to identify major peaks. The possible relationship between UPLC fingerprints and anti-HIV 1 activities of Rheum species were deduced by mathematical statistics method.RESULT:
Samples of R. austral, R. austral, R. hotaoense exhibited good anti-HIV 1 activities with IC50 < or = 0.2 mg x L(-1). The correlation of anti-HIV 1 activities and fingerprints showed that three compounds were the main bioactive components, and their retention times were 4.74, 7.99, 21.18 min, respectively.CONCLUSION:
Compounds in Rheum species with possible anti-HIV 1 activities were deduced by spectrum-effect relationship study. This study supported for study of medicinal plants in Rheum.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rheum
/
Medicamentos de Ervas Chinesas
/
HIV-1
/
Fármacos Anti-HIV
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article