A cell-based reporter assay for screening for EcR agonist/antagonist activity of natural ecdysteroids in Lepidoptera (Bm5) and Diptera (S2) cell cultures, followed by modeling of ecdysteroid-EcR interactions and normal mode analysis.

Zotti, Moisés J; De Geyter, Ellen; Swevers, Luc; Braz, Antônio S K; Scott, Luis P B; Rougé, Pierre; Coll, Josep; Grutzmacher, Anderson D; Lenardão, Eder J; Smagghe, Guy

Zotti, Moisés J; De Geyter, Ellen; Swevers, Luc; Braz, Antônio S K; Scott, Luis P B; Rougé, Pierre; Coll, Josep; Grutzmacher, Anderson D; Lenardão, Eder J; Smagghe, Guy.

Afiliação

Zotti MJ; Department of Crop Protection, Ghent University, Coupure links 653, 9000 Ghent, Belgium; Department of Phytosanitary, FAEM, Federal University of Pelotas, P.O. Box 354, CEP, 96010-900 Pelotas, RS, Brazil; Department of Crop Protection, Federal University of Santa Maria, Santa Maria, Brazil. Electronic address: moises.zotti@ugent.be.

Pestic Biochem Physiol ; 107(3): 309-20, 2013 Nov.

Article em En | MEDLINE | ID: mdl-24267692

Assuntos

Ecdisteroides/metabolismo; Lepidópteros/metabolismo; Receptores de Esteroides/metabolismo; Animais; Linhagem Celular; Dípteros; Ligação Proteica; Transdução de Sinais

Palavras-chave

Cell-based screening system; Ecdysteroid agonist; Ecdysteroid antagonist; Molecular modeling; Normal mode analysis; Steroids

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores de Esteroides / Ecdisteroides / Lepidópteros Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals Idioma: En Ano de publicação: 2013 Tipo de documento: Article

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