MALDI imaging mass spectrometry in glomerulonephritis: feasibility study.
Histopathology
; 64(6): 901-6, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24279679
ABSTRACT
AIMS:
The in-situ proteomics technology known as matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a powerful technique that combines traditional histology and proteomics. METHODS ANDRESULTS:
MALDI-IMS was applied to routine diagnostic kidney biopsies in a small group of cases of membranous glomerulonephritis and minimal change disease. Molecular changes were observed not only in the tissue areas with pathological alterations, but also in morphologically normal-looking tissue, highlighting the potential feasibility of using MALDI-IMS as a tool in nephropathology.CONCLUSIONS:
This technology can be applied to any biopsy where a frozen section is obtained as part of the diagnostic process. Although we do not yet know the molecular identity of the differentially expressed proteins/peptides, they could represent powerful classifiers of nosological groups.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Glomerulonefrite
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article