Comparative proteomics reveals association of early accumulated proteins in conferring butachlor tolerance in three N(2)-fixing Anabaena spp.

ABSTRACT

Butachlor an extensively used rice field herbicide negatively affects the cyanobacterial proliferation, yet the molecular mechanism underlying its toxicity in diazotrophic cyanobacteria is largely unknown. The present study focuses on the comparative proteomics to decode the molecular basis of butachlor toxicity/tolerance in three Anabaena species e.g. Anabaena sp. PCC 7120, Anabaena doliolum and Anabaena L31. 75 differentially expressed proteins from each Anabaena sp. included those involved in photosynthesis, C, N and protein metabolism, redox homeostasis, and signal transduction. While early accumulated proteins related to photosynthesis (atpA, atpB), carbon metabolism (glpx, fba and prk), protein folding (groEL, PPIase), regulation (orrA) and other function (OR, akr) appeared crucial for tolerance of Anabaena L31, the late accumulated proteins in Anabaena 7120 presumably offer acclimation during prolonged exposure to butachlor. Contrary to the above, a multitude of down-accumulated proteins vis-a-vis metabolisms augment sensitivity of A. doliolum to butachlor. A cluster of high abundant proteins (atpA, groEL, OR, AGTase, Alr0803, Alr0806, Alr3090, Alr3199, All4050 and All4051) common across the three species may be taken as markers for butachlor tolerance and deserve exploitation for stress management and transgenic development. BIOLOGICAL SIGNIFICANCE: Cyanobacteria offer an eco-friendly alternative to chemical fertilizers for increasing productivity, especially in rice cultivation. This study is the first to compare the proteome of three diazotrophic cyanobacteria subjected to butachlor, a pre-emergent herbicide extensively used in rice paddy. Changes in protein dynamics over time along with physiological and biochemical attributes clearly provide a comprehensive overview on differential tolerance of Anabaena species to butachlor. Molecular docking further added a new dimension in identification of potential protein candidates for butachlor stress management in cyanobacteria. This study strongly recommends combined application of Anabaena spp. A. L31 and A. PCC7120 as biofertilizer in paddy fields undergoing butachlor treatment.