Chemically sulfated galactomannan from Dimorphandra gardneriana seed: characterization and toxicity evaluation.
Carbohydr Polym
; 101: 1013-7, 2014 Jan 30.
Article
em En
| MEDLINE
| ID: mdl-24299869
ABSTRACT
Dimorphandra gardneriana galactomannan (DG) was sulfated in pyridineformamide using chlorosulfonic acid as the sulfation agent. The degree of substitution was 0.32, determined from the sulfur percentage. Confirmation of sulfation was obtained by FTIR spectroscopy through the presence of an asymmetrical SO stretching vibration at 1,259 cm(-1). NMR data showed that the sulfation occurred on primary hydroxyl groups. NMR and GPC data indicate degradation during reaction with elimination of galactose. At the maximum tested concentration of 1,000 µg/mL, unmodified DG polysaccharide did not show a statistically significant cytotoxicity in Vero cells by the MTT method. Therefore, the CC50>1,000 µg/mL obtained for the sulfated polysaccharides from D. gardneriana in Vero cells point to its lower cytotoxicity than the sulfated galactomannan from Mimosa scabrella.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfatos
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Testes de Toxicidade
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Fabaceae
/
Mananas
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article