Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain.
Biotechnol Lett
; 36(4): 813-9, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24322771
ABSTRACT
Many γ-butyrolactone-autoregulator receptors control the production of secondary metabolites in Streptomyces spp. Hence, AvaR1, an autoregulator receptor protein in Streptomyces avermitilis, was characterized as a negative regulator of avermectin (Ave) production. Deletion of AvaR1 in a high-producing strain increased production of Ave B1a approx. 1.75 times (~700 µg/ml) compared with the parent strain. Semi-quantitative RT-PCR and electrophoretic mobility shift assays revealed that AvaR1 regulates the biosynthesis of Ave but not through the aveR pathway-specific regulatory gene. A special signaling molecule, avenolide, increased production of Ave. This study has refined our understanding of how avenolide regulates the production of Aves which is promising for developing new methods to improve the production of antibiotics in industrial strains.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
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Streptomyces
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Proteínas de Bactérias
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Ivermectina
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Regulação Bacteriana da Expressão Gênica
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article