[Antimicrobial resistance and virulence characteristics of Vibrio parahaemolyticus].
Zhonghua Yi Xue Za Zhi
; 93(34): 2733-5, 2013 Sep 10.
Article
em Zh
| MEDLINE
| ID: mdl-24360109
ABSTRACT
OBJECTIVE:
To explore drug resistance and virulence characteristics of clinically isolated Vibrio parahaemolyticus (VP).METHODS:
The patient records were reviewed. The laboratory information system was employed to count the quantity of bacterial diarrhea pathogens at our intestinal clinic from 2009 to 2012. The drug resistant phenotype was tested by the method of disk diffusion. Polymerase chain reaction (PCR) was used to detect thermo-stable direct hemolysin (TDH) gene (tdh), TDH-related hemolysin gene (trh) and type III secretion system 2 (T3SS2α, T3SS2ß).RESULTS:
The VP infection rate was 37.90% (274/723). Susceptibility test showed that the resistance rates of ampicillin, cefalotin, cefuroxime and amikacin were 100% (216/216), 44.91% (97/216), 24.07% (52/216), 9.26% (20/216). The other 11 kinds of antibiotics were sensitive or intermediate. 96.76% (209/216) VP harbored tdh gene, 1.39% (3/216) trh gene and 100% T3SS2 gene, with 213 strains T3SS2α positive, 2 strains T3SS2ß positive and 1 strain both T3SS2α and T3SS2ß positive.CONCLUSION:
The resistant rate of VP to ampicillin is the highest and VP has different levels of resistance against other antibiotics. Most clinical isolates of VP harbor tdh gene and T3SS2 plays an important role in the pathogenicity of VP.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vibrio parahaemolyticus
/
Farmacorresistência Bacteriana
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article