Analytical determination of flavonoids aimed to analysis of natural samples and active packaging applications.
Food Chem
; 150: 119-27, 2014 May 01.
Article
em En
| MEDLINE
| ID: mdl-24360428
ABSTRACT
Several HPLC and UHPLC developed methods were compared to analyse the natural antioxidants catechins and quercetin used in active packaging and functional foods. Photodiode array detector coupled with a fluorescence detector and compared with LTQ-Orbitrap-MS was used. UHPLC was investigated as quick alternative without compromising the separation, analysis time shortened up to 6-fold. The feasibility of the four developed methods was compared. Linearity up to 0.9995, low detection limits (between 0.02 and 0.7 for HPLC-PDA, 2 to 7-fold lower for HPLC- LTQ-Orbitrap-MS and from 0.2 to 2mgL(-)(1) for UHPLC-PDA) and good precision parameters (RSD lower than 0.06%) were obtained. All methods were successfully applied to natural samples. LTQ-Orbitrap-MS allowed to identify other analytes of interest too. Good feasibility of the methods was also concluded from the analysis of catechin and quercetin release from new active packaging materials based on polypropylene added with catechins and green tea.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plásticos
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Quercetina
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Cacau
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Extratos Vegetais
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Catequina
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Embalagem de Alimentos
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Camellia sinensis
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Antioxidantes
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article