Screening and analysis of metabolites in rat urine after oral administration of Apocynum venetum L. extracts using HPLC-TOF-MS.
J Sep Sci
; 37(5): 515-26, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24376178
ABSTRACT
HPLC with diode array detection and ESI-TOF-MS was used for the study of the constituents in Apocynum venetum L. extracts and the metabolites in rat urine after oral administration of A. venetum L. extracts. A formula database of the known constituents in A. venetum L. was established, and 21 constituents were rapidly identified by accurately matching their molecular masses with the formulae of the compounds in the database. Furthermore, 34 metabolites were detected and elucidated in the rat urine. The scientific and plausible biotransformation pathways of the flavonoid components in A. venetum L. were also proposed together with the presentation of clues for potential mechanisms of bioactivity. This specific and sensitive HPLC-ESI-TOF-MS method can be used to identify the chemical components in the extracts of A. venetum L. and their metabolites in rat urine. This method can also be used to reveal the possible metabolic mechanisms of action of the extract components in vivo.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Urina
/
Medicamentos de Ervas Chinesas
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Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas por Ionização por Electrospray
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Apocynum
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article