[Cryopreservation of bone marrow for the autologous transplantation in children]. / Kryokonservierung von Knochenmark zur autologen Transplantation bei Kindern.
Folia Haematol Int Mag Klin Morphol Blutforsch
; 114(1): 62-77, 1987.
Article
em De
| MEDLINE
| ID: mdl-2439419
ABSTRACT
In preparing the autologous transplantation of children a method for cryoconservation of bone-marrow was developed by means of investigating the donor's bone-marrow. This method is adapted to our conditions, can easily be practised and is cell-preserving. Quantity and quality of the stored bone-marrow cells were evaluated concerning their proliferation capability by means of CFU-c assays. The highest recovery in CFU-c (78%) and cells (98%) was observed if isolated mononuclear cells with cryoprotective addition of 5% DMSO, 20% of human albumin, and 20% of serum were slowly frozen at a controllable rate, stored in liquid oxygen and thawed very quickly. According to the elaborated method the remission marrow was taken from 15 children affected with malignant diseases for autologous reinfusion. The data gained here confirm the experimental experiences.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação Biológica
/
Transplante de Medula Óssea
Limite:
Adolescent
/
Child
/
Child, preschool
/
Humans
Idioma:
De
Ano de publicação:
1987
Tipo de documento:
Article