Selective irreversible chemical tagging of cysteine with 3-arylpropiolonitriles.
Bioconjug Chem
; 25(2): 202-6, 2014 Feb 19.
Article
em En
| MEDLINE
| ID: mdl-24410136
ABSTRACT
Exquisite chemoselectivity for cysteine has been found for a novel class of remarkably hydrolytically stable reagents, 3-arylpropiolonitriles (APN). The efficacy of the APN-mediated tagging was benchmarked against other cysteine-selective methodologies in a model study on a series of traceable amino acid derivatives. The selectivity of the methodology was further explored on peptide mixtures obtained by trypsin digestion of lysozyme. Additionally, the superior stability of APN-cysteine conjugates in aqueous media, human plasma, and living cells makes this new thiol-click reaction a promising methodology for applications in bioconjugation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cisteína
/
Nitrilas
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article