Electrochemiluminescence aptasensor based on bipolar electrode for detection of adenosine in cancer cells.
Biosens Bioelectron
; 55: 459-63, 2014 May 15.
Article
em En
| MEDLINE
| ID: mdl-24441543
ABSTRACT
Here we report a novel approach for the detection of adenosine in cancer cells by electrochemiluminescence (ECL) on a wireless indium tin oxide bipolar electrode (BPE). In this approach, ferrocene (Fc) which is labeled on adenosine aptamer is enriched on one pole of the BPE by hybridization with its complementary DNA (ssDNA) and oxidized to Fc(+) under an external voltage of 5.0V at the two ends of BPE. Then, a reversed external voltage was added on the BPE, making Fc(+) enriched pole as cathode. The presence of Fc(+) promotes the oxidation reaction on the anodic pole of the BPE, resulting in a significant increase of ECL intensity using Ru(bpy)3(2+)/tripropylamine (TPA) system as test solution. The presence of target adenosine was reflected by the ECL signal decrease on the anodic pole caused by the target-induced removal of ferrocene-aptamer on the cathodic pole. The decrease of ECL signal was logarithmically linear with the concentration of ATP in a wide range from 1.0 fM to 0.10 µM. This ECL biosensing system could accurately detect the level of adenosine released from cancer cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Biomarcadores Tumorais
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Adenosina
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Condutometria
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Eletrodos
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Aptâmeros de Nucleotídeos
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Medições Luminescentes
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article