Your browser doesn't support javascript.
loading
EGF receptor-dependent mechanism may be involved in the Tamm-Horsfall glycoprotein-enhanced PMN phagocytosis via activating Rho family and MAPK signaling pathway.
Li, Ko-Jen; Siao, Sue-Cien; Wu, Cheng-Han; Shen, Chieh-Yu; Wu, Tsai-Hung; Tsai, Chang-Youh; Hsieh, Song-Chou; Yu, Chia-Li.
Afiliação
  • Li KJ; Institute of Clinical Medicine, National Yang-Ming University College of Medicine, Taipei 11221, Taiwan. dtmed170@yahoo.com.tw.
  • Siao SC; Institute of Molecular Medicine, National Taiwan University College of Medicine, Taipei 10002, Taiwan. syuecian@gmail.com.
  • Wu CH; Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei 10002, Taiwan. chenghanwu@ntu.edu.tw.
  • Shen CY; Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei 10002, Taiwan. tsichhl@gmail.com.
  • Wu TH; Section of Nephrology, Taipei Veterans General Hospital, Taipei 11221, Taiwan. wuth@vghtpe.gov.tw.
  • Tsai CY; Section of Allergy, Immunology & Rheumatology, Taipei Veterans General Hospital, Taipei 11221, Taiwan. cytsai@vghtpe.gov.tw.
  • Hsieh SC; Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 10002, Taiwan. hsiehsc@ntu.edu.tw.
  • Yu CL; Institute of Molecular Medicine, National Taiwan University College of Medicine, Taipei 10002, Taiwan. chialiyu@ntu.edu.tw.
Molecules ; 19(1): 1328-43, 2014 Jan 21.
Article em En | MEDLINE | ID: mdl-24451252
Our previous studies showed that urinary Tamm-Horsfall glycoprotein (THP) potently enhanced polymorphonuclear neutrophil (PMN) phagocytosis. However, the domain structure(s), signaling pathway and the intracellular events responsible for THP-enhanced PMN phagocytosis remain to be elucidated. THP was purified from normal human urine. The human promyelocytic leukemia cell line HL-60 was induced to differentiate into PMNs by all-trans retinoid acid. Pretreatment with different MAPK and PI3K inhibitors was used to delineate signaling pathways in THP-enhanced PMN phagocytosis. Phosphorylation of molecules responsible for PMN phagocytosis induced by bacterial lipopolysaccharide (LPS), THP, or human recombinant epidermal growth factor (EGF) was evaluated by western blot. A p38 MAPK inhibitor, SB203580, effectively inhibited both spontaneous and LPS- and THP-induced PMN phagocytosis. Both THP and LPS enhanced the expression of the Rho family proteins Cdc42 and Rac that may lead to F-actin re-arrangement. Further studies suggested that THP and EGF enhance PMN and differentiated HL-60 cell phagocytosis in a similar pattern. Furthermore, the EGF receptor inhibitor GW2974 significantly suppressed THP- and EGF-enhanced PMN phagocytosis and p38 and ERK1/2 phosphorylation in differentiated HL-60 cells. We conclude that EGF receptor-dependent signaling may be involved in THP-enhanced PMN phagocytosis by activating Rho family and MAP kinase.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fagocitose / Proteínas rho de Ligação ao GTP / Sistema de Sinalização das MAP Quinases / Uromodulina / Receptores ErbB / Neutrófilos Limite: Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fagocitose / Proteínas rho de Ligação ao GTP / Sistema de Sinalização das MAP Quinases / Uromodulina / Receptores ErbB / Neutrófilos Limite: Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article