Initiation of the flexirubin biosynthesis in Chitinophaga pinensis.
Microb Biotechnol
; 7(3): 232-41, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24467333
ABSTRACT
Bacteria from the Bacteroidetes phylum are known producers of the chemotaxonomic relevant flexirubins. These orange pigments comprise a non-isoprenoid aryl-polyene carboxylic acid esterified with a dialkylresorcinol. Herein, we report a gene cluster from Chitinophaga pinensis encoding the biosynthesis of the polyene moiety and the biochemical characterization of a tyrosine ammonia-lyase and a 4-coumarate-CoA ligase responsible for the initiation of the polyene biosynthesis. Additionally, the flexirubin of C. pinensis was characterized by a combination of feeding experiments, high-performance liquid chromatography tandem mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polienos
/
Bacteroidetes
/
Vias Biossintéticas
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article