Acetylcholinesterase inhibitory activity of Haplophyton cimicidum.
Nat Prod Res
; 28(10): 757-9, 2014.
Article
em En
| MEDLINE
| ID: mdl-24484055
ABSTRACT
In Oaxaca, México, Haplophyton cimicidum is used in combination with other plants as part of a folk strategy against arthropod pests of crops and cattle. Methanolic and crude alkaloid (acid-base extraction) extracts of H. cimicidum leaves were analysed by thin-layer chromatography (TLC) and assayed in vitro for acetylcholinesterase (AChE) inhibitory activity using Spodoptera frugiperda homogenates as source of enzyme. Derivatised chromatographic plates indicated the presence of indoles and alkaloids in the extracts. The crude alkaloid extract exhibited a higher number of compounds than the methanolic extract as judged by the number of spots on TLC plates. The crude alkaloid extract had a weak inhibition potential of AChE with a lower IC50 (93 µg mL(-1)) than the methanolic extract (159 µg mL(-1)). Indole alkaloids may be responsible for the activity, though a subsequent analysis of the extract components is necessary to determine the active alkaloid(s).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibidores da Colinesterase
/
Apocynaceae
/
Alcaloides Indólicos
Limite:
Animals
País/Região como assunto:
Mexico
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article