Protective effects of aliskiren on atrial ionic remodeling in a canine model of rapid atrial pacing.
Cardiovasc Drugs Ther
; 28(2): 137-43, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24488001
ABSTRACT
PURPOSE:
Aliskiren inhibits the activation of the renin-angiotensin system. Here, we investigated the effects of aliskiren on chronic atrial iron remodeling in the experimental canine model of rapid atrial pacing.METHODS:
Twenty-eight dogs were assigned to sham (S), control paced (C), paced + aliskiren (10 mg Kg(-1) d(-1), A1), and paced + aliskiren (20 mg Kg(-1) d(-1), A2) groups. Rapid atrial pacing at 500 bpm was maintained for 2 weeks, while group S was not paced. Levels of serum angiotensin-converting enzyme and angiotensin II after pacing were determined by ELISA. Whole-cell patch-clamp technique, western blot, and RT-PCR were applied to assess atrial ionic remodeling.RESULTS:
The density of I CaL and I Na currents (pA/pF) was significantly lower in group C compared with group S (I CaL -4.09 ± 1.46 vs. -6.12 ± 0.58,P < 0.05; I Na 30.48 ± 6.08 vs. 46.31 ± 4.73, P < 0.05). However, the high dose of aliskiren elevated the density of I CaL and I Na currents compared with group C (I CaL -6.23 ± 1.35 vs. -4.09 ± 1.46, P < 0.05; I Na 58.62 ± 16.17 vs. 30.48 ± 6.08, P < 0.01). The relative mRNA and protein expression levels of Cav1.2 and Nav1.5α were downregulated in group C respectively (Cav1.2 0.46 ± 0.08; Nav1.5α 0.52 ± 0.08, P < 0.01; Cav1.2 0.31 ± 0.03; Nav1.5α 0.41 ± 0.04, P < 0.01;), but were upregulated by aliskiren.CONCLUSIONS:
Aliskiren has protective effects on atrial tachycardia-induced atrial ionic remodeling.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Remodelamento Atrial
/
Fumaratos
/
Amidas
/
Átrios do Coração
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article