Your browser doesn't support javascript.
loading
Expression and co-expression of surface markers of pluripotency on human amniotic cells cultured in different growth media.
Bryzek, Aleksandra; Czekaj, Piotr; Plewka, Danuta; Komarska, Halina; Tomsia, Marcin; Lesiak, Marta; Sieron, Aleksander L; Sikora, Jerzy; Kopaczka, Katarzyna.
Afiliação
  • Bryzek A; Department of Cytophysiology, Medical University of Silesia, Katowice, Poland.
  • Czekaj P; Department of Cytophysiology, Medical University of Silesia, Katowice, Poland.
  • Plewka D; Department of Cytophysiology, Medical University of Silesia, Katowice, Poland.
  • Komarska H; Department of Molecular Biology, Medical University of Silesia, Katowice, Poland.
  • Tomsia M; Department of Cytophysiology, Medical University of Silesia, Katowice, Poland.
  • Lesiak M; Department of Molecular Biology, Medical University of Silesia, Katowice, Poland.
  • Sieron AL; Department of Molecular Biology, Medical University of Silesia, Katowice, Poland.
  • Sikora J; Department of Perinatology and Gynecology, Central Clinical Hospital, Medical University of Silesia, Katowice, Poland.
  • Kopaczka K; Students Scientific Society, Medical University of Silesia, Katowice, Poland.
Ginekol Pol ; 84(12): 1012-24, 2013 Dec.
Article em En | MEDLINE | ID: mdl-24505948
OBJECTIVES: Despite constant advances in the field of biology and medical application of human embryonic stem cells, the molecular mechanism of pluripotency remains largely unknown. So far, definitions of pluripotent stem cells (SC) have been based on a limited number of antigenic markers and have not allowed for unambiguous determination of the homogeneity of each subpopulation. Moreover, the use of some crucial pluripotency markers such as SSEA-3 and SSEA-4 has recently been questioned due to the possibility that the pattern of surface glycans may be changed depending on the content of the cell culture medium. AIM: Quantitative analysis of amniotic SC subpopulations cultured in different media, based on the following pluripotency surface markers: SSEA-3, SSEA-4, TRA- 1-60 and TRA- 1-81 expression and co-expression. MATERIAL AND METHODS: Immunofluorescence and fluorescence microscopy were used to identify and localize SC within a normal human placenta at term. The number of SSEA-4+, SSEA-3+, TRA-1-60+ and TRA-1-81+ cells and cells with co-expression of the above mentioned markers, cultured in media containing different protein supplements of animal origin, was counted by flow cytometry RESULTS AND CONCLUSIONS: Cells with characteristics of embryonic SC were identified in the amniotic epithelium and the chorion, but not in the decidua basalis. Amniotic epithelium contained various types of SC, with SSEA-4+ as the most numerous. Disproportion in the number of SSEA-4+, SSEA-3+, TRA-1-60+ and TRA-1-81+ cells and cells characterized by co-expression of these antigens, as well as lack of quantitative differences between SC subpopulations cultured in different media, was observed. In conclusion, the amniotic epithelium is composed of SC at different stages of the development but human amnion might become an alternative source of SSEA-4+ embryonic-like SC. The composition of the evaluated media, characterized by different content of animal-derived proteins, does not influence the number of cells identified within the SC subpopulations.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos Glicosídicos Associados a Tumores / Células-Tronco Pluripotentes / Antígenos Embrionários Estágio-Específicos / Líquido Amniótico Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Animals / Female / Humans / Pregnancy Idioma: En Ano de publicação: 2013 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos Glicosídicos Associados a Tumores / Células-Tronco Pluripotentes / Antígenos Embrionários Estágio-Específicos / Líquido Amniótico Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Animals / Female / Humans / Pregnancy Idioma: En Ano de publicação: 2013 Tipo de documento: Article