Isolation of single-base genome-edited human iPS cells without antibiotic selection.
Nat Methods
; 11(3): 291-3, 2014 Mar.
Article
em En
| MEDLINE
| ID: mdl-24509632
ABSTRACT
Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma Humano
/
Técnicas Citológicas
/
Células-Tronco Pluripotentes Induzidas
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article