Development of an in vitro fibrin clot model to evaluate fibrinolytic agents for wound care application.
J Wound Care
; 23(2): 66-7, 70, 72, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-24526082
ABSTRACT
OBJECTIVE:
To describe an in vitro fibrin clot model that could reliably assess the fibrinolytic activity of enzymatic debriding agents for wound care application.METHOD:
A model of a fibrin clot was reconstructed in vitro by mixture of human fibrinogen and (alpha)-thrombin supplemented with factor XIII. These clots were then treated with enzymatic ointments. Fibrinolytic activity was investigated by measuring D-dimer levels, using an automated immunoturbidimetric Liatest D-dimer assay.RESULTS:
Collagenase and papain-urea ointments demonstrated fibrinolytic activity which was macroscopically visible. Their effect was identical on the in vitro reconstructed fibrin clot and ex vivo collected wound fibrin clot; collagenase and papain-urea both induced a complete degradation and dissolution of both fibrin clots after 24 hours of treatment. This was associated with an increase in D-dimer concentration.CONCLUSION:
This reconstructed fibrin clot in vitro model has the potential to predict the efficacy of fibrinolytic agents and therefore appears to be a suitable model for in vitro assays. DECLARATION OF INTEREST This study was supported by a grant from URGO Laboratory.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ureia
/
Cicatrização
/
Fibrina
/
Papaína
/
Colagenases
/
Fibrinólise
/
Fibrinolíticos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article