Detection of human immunodeficiency virus and other retroviruses in cell culture supernatants by a reverse transcriptase microassay.
J Virol Methods
; 19(2): 161-8, 1988 Feb.
Article
em En
| MEDLINE
| ID: mdl-2452827
A micromethod for the detection of human immunodeficiency virus (HIV) and other retroviruses in cell culture supernatants is described which applies a DEAE ion exchanger for recovery of polynucleotides synthesized in vitro by the retroviral reverse transcriptase. Cell culture, sample preparation, and test performance including the washing step are adapted to microtitre plates. Compared to the standard method this technique produced less non-specific reactions, resulting in a more than 3-fold higher sensitivity, a higher reproducibility due to lower intrarun variations and allowed an increase in the daily sample accomplishment per person 3- to 4-fold at lower costs per sample.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retroviridae
/
HIV
/
DNA Polimerase Dirigida por RNA
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1988
Tipo de documento:
Article