Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics.
Proc Natl Acad Sci U S A
; 111(9): 3419-24, 2014 Mar 04.
Article
em En
| MEDLINE
| ID: mdl-24550488
ABSTRACT
Transcription factors IIS (TFIIS) and IIF (TFIIF) are known to stimulate transcription elongation. Here, we use a single-molecule transcription elongation assay to study the effects of both factors. We find that these transcription factors enhance overall transcription elongation by reducing the lifetime of transcriptional pauses and that TFIIF also decreases the probability of pause entry. Furthermore, we observe that both factors enhance the processivity of RNA polymerase II through the nucleosomal barrier. The effects of TFIIS and TFIIF are quantitatively described using the linear Brownian ratchet kinetic model for transcription elongation and the backtracking model for transcriptional pauses, modified to account for the effects of the transcription factors. Our findings help elucidate the molecular mechanisms by which transcription factors modulate gene expression.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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RNA Polimerases Dirigidas por DNA
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RNA Mensageiro
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Regulação da Expressão Gênica
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Fatores de Transcrição TFII
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Fatores de Elongação da Transcrição
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Elongação da Transcrição Genética
Tipo de estudo:
Health_economic_evaluation
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article