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Mitochondrial oxidative stress-induced epigenetic modifications in pancreatic epithelial cells.
Mishra, Pradyumna Kumar; Raghuram, Gorantla Venkata; Jain, Deepika; Jain, Subodh Kumar; Khare, Naveen Kumar; Pathak, Neelam.
Afiliação
  • Mishra PK; Translational Research Lab, School of Biological Sciences, Dr Hari Singh Gour Central University, Sagar, Madhya Pradesh, India. Email: pkm_8bh@yahoo.co.uk.
Int J Toxicol ; 33(2): 116-29, 2014.
Article em En | MEDLINE | ID: mdl-24563415
Emerging studies have linked prooxidative carbamate compound exposures with various human pathologies including pancreatic cancer. In these studies, our aim was to examine mitochondrial oxidative stress-mediated aberrant chromatin responses in human pancreatic ductal epithelial cells. Posttranslational histone modifications, promoter DNA methylation, and micro-RNA (miRNA) expression patterns were evaluated following induction of mitochondrial oxidative stress by N-succinimidyl N-methylcarbamate exposure. In treated cells, perturbation in mitochondrial machinery led to hypermethylation of p16 and smad4 gene promoters and downregulation of respective gene products. Posttranslational histone modifications that include hypoacetylation of acetylated histone (AcH) 3 and AcH4, hypermethylation of monomethylated histone 3 at lysine 9 and trimethylated histone 4 at lysine 20 ubiquitinated histone (uH) 2A/uH2B, and increased phosphorylation of H2AX and H3 were observed in the treated cells. Altered expression of miRNAs denoted possible location of corresponding genes at oxidatively damaged fragile sites. Collectively, our results provide a direct role of mitochondrial oxidative stress-mediated epigenetic imbalance to perturbed genomic integrity in oxygen radical-induced pancreatic injury. Further, identification and characterization of molecular switches that affect these epigenomic signatures and targets thereof will be imperative to understand the complex role of redox-regulatory network in pancreatic milieu.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pâncreas / Estresse Oxidativo / Epigênese Genética / Células Epiteliais / Mitocôndrias Limite: Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pâncreas / Estresse Oxidativo / Epigênese Genética / Células Epiteliais / Mitocôndrias Limite: Humans Idioma: En Ano de publicação: 2014 Tipo de documento: Article