A new modified cytosine base capable of base pairing with guanine using four hydrogen bonds.
Org Biomol Chem
; 12(14): 2255-62, 2014 Apr 14.
Article
em En
| MEDLINE
| ID: mdl-24569493
ABSTRACT
Oligonucleotides, containing 4-N-(1H-pyrrol-2-ylcarbonyl)deoxycytidine (dC(Pyc)) and related derivatives, were synthesized via deprotection using 1.5 M NaOMe/MeOH. Among them, oligodeoxynucleotides containing dC(Pyc) exhibited a higher hybridization affinity for DNA and RNA than the unmodified oligodeoxynucleotides. Comparative analysis between dC(Pyc) and its derivatives by molecular dynamic simulation indicated that the C(Pyc) residue could form four hydrogen bonds with the opposite G nucleobase keeping a more planar structure than the C(Inc) residue where the Pyc group was replaced with a 1H-indol-2-ylcarbonyl group.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Citosina
/
Guanina
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article