Partial somatic to stem cell transformations induced by cell-permeable reprogramming factors.
Sci Rep
; 4: 4361, 2014 Mar 12.
Article
em En
| MEDLINE
| ID: mdl-24618595
ABSTRACT
The production of pluripotent stem cells (iPSCs) for therapeutic applications will require practical methods to achieve tight temporal and quantitative control of reprogramming factor (RF) expression, while avoiding the mutagenic potential of gene transfer. Toward this end, we have developed cell-permeable RF proteins (CP-RFs) incorporating newly developed macromolecule transduction domains (MTDs). Treatment of human dermal fibroblasts (HDFs) with combinations of cell-permeable OCT4, SOX2, KLF4, CMYC and either NANOG or LIN28 proteins induced the outgrowth of stem cell-like colonies (iSCs). iSC colonies generated with CP-RFs resembled embryonic stem cells with regard to morphology, biomarker expression, and extended capacity for self-renewal, but failed to expand as iPSC or ES cell lines. Partial reprogramming appears to be a common response to protein-based delivery of programming factors into somatic cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Proteínas Recombinantes de Fusão
/
Reprogramação Celular
/
Peptídeos Penetradores de Células
/
Fibroblastos
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article