The use of amphipols for solution NMR studies of membrane proteins: advantages and constraints as compared to other solubilizing media.
J Membr Biol
; 247(9-10): 827-42, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-24676477
ABSTRACT
Solution-state nuclear magnetic resonance studies of membrane proteins are facilitated by the increased stability that trapping with amphipols confers to most of them as compared to detergent solutions. They have yielded information on the state of folding of the proteins, their areas of contact with the polymer, their dynamics, water accessibility, and the structure of protein-bound ligands. They benefit from the diversification of amphipol chemical structures and the availability of deuterated amphipols. The advantages and constraints of working with amphipols are discussed and compared to those associated with other non-conventional environments, such as bicelles and nanodiscs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Tensoativos
/
Espectroscopia de Ressonância Magnética
/
Membrana Celular
/
Bicamadas Lipídicas
/
Proteínas de Membrana
Tipo de estudo:
Health_technology_assessment
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article