Staphylococcal enterotoxin B suppresses Alix and compromises intestinal epithelial barrier functions.
J Biomed Sci
; 21: 29, 2014 Apr 09.
Article
em En
| MEDLINE
| ID: mdl-24712823
ABSTRACT
BACKGROUND:
The epithelial barrier dysfunction plays a critical role in the pathogenesis of a broad array of immune diseases. Alix protein is involved in the endolysosome system. This study aims to elucidate the role of Alix in the maintenance of epithelial barrier function.RESULTS:
The results showed that Alix was detected in T84 cells at both mRNA and protein levels. Exposure to Staphylococcal enterotoxin B (SEB) markedly suppressed the expression of Alix in T84 cells, which could be blocked by knocking down the Toll like receptor 2. The exposure to SEB did not affect the TER, but markedly increased the permeability of T84 monolayers to OVA; the OVA passing through T84 monolayers still preserved the antigenicity manifesting inducing antigen specific T cells proliferation.CONCLUSIONS:
Alix protein plays a critical role in the maintenance of the barrier function of T84 monolayers.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação ao Cálcio
/
Proteínas de Ciclo Celular
/
Enterotoxinas
/
Receptor 2 Toll-Like
/
Complexos Endossomais de Distribuição Requeridos para Transporte
/
Doenças do Sistema Imunitário
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article