MicroRNA-155 inhibits angiotensin II-induced vascular smooth muscle cell proliferation.
J Renin Angiotensin Aldosterone Syst
; 15(2): 109-16, 2014 Jun.
Article
em En
| MEDLINE
| ID: mdl-24737641
ABSTRACT
BACKGROUND:
MicroRNA-155 (miR-155) is a multifunctional signal microRNA that participates in a variety of cardiovascular diseases and is involved in physiological and pathological processes in different cell types.OBJECTIVE:
The objective of this article is to examine the effect of miR-155 on angiotensin II (Ang II)-induced primary mice vascular smooth muscle cell (VSMC) proliferation.METHODS:
Primary cultured VSMCs from the aorta of C57/BL6 mice were incubated with Ang II and miR-155. Cells were counted using CCK-8 and EdU, and flow cytometric analysis of cell cycle progression was performed. Angiotensin II 1 type receptor (AT1R) gene and protein expression were measured by real-time polymerase chain reaction and Western blotting.RESULTS:
1) Ang II increased the viability of VSMCs in a dose- and time-dependent manner. 2) miR-155 opposed the Ang II-induced increase in VSMC viability. 3) miR-155 inhibited Ang II-induced proliferation of VSMCs. 4) miR-155 increased the number of VSMCs in the G1 phase compared to G2 and M cell cycle phases. 5) miR-155 decreased ATR1 gene and protein expression.CONCLUSION:
miR-155 downregulation of Ang II-induced VSMC viability identifies it as an important regulator of cell proliferation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Angiotensina II
/
MicroRNAs
/
Proliferação de Células
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Antagonistas de Receptores de Angiotensina
/
Músculo Liso Vascular
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article