False positivity of ETV6/RUNX1 detected by FISH in healthy newborns and adults.
Pediatr Blood Cancer
; 61(9): 1704-6, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-24740533
ABSTRACT
The leukemia-associated ETV6-RUNX1-translocation frequently emerges prenatally. Reverse-transcriptase PCR screening may indicate presence of ETV6-RUNX1 transcripts in random cord blood samples. Subsequent cell enrichment validation finds significantly lower levels than validation applying fluorescence in situ hybridization (FISH) (<10(-5) vs. 10(-3) to 10(-4)). Using three FISH probe sets, we screened 179,000 cells from ETV6-RUNX1-positive dilution series, healthy adults and random cord blood samples. The t(12;21) single fusion extra signal translocation probe and the ETV6 break apart probe gave false positive results mimicking ETV6-RUNX1-positive cell levels of 10(-3). This questions the paradigm that 1% of newborns have ETV6-RUNX1-positive cells at levels of 10(-3) to 10(-4).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Translocação Genética
/
Proteínas de Fusão Oncogênica
/
Subunidade alfa 2 de Fator de Ligação ao Core
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Humans
/
Newborn
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article