A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification.
Chem Sci
; 4(9): 3455-3458, 2013 Jul 29.
Article
em En
| MEDLINE
| ID: mdl-24741436
ABSTRACT
Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article