Efficient molecular marker design using the MaizeGDB Mo17 SNPs and Indels track.
G3 (Bethesda)
; 4(6): 1143-5, 2014 Apr 17.
Article
em En
| MEDLINE
| ID: mdl-24747759
ABSTRACT
Positional cloning in maize (Zea mays) requires development of markers in the region of interest. We found that primers designed to amplify annotated insertion-deletion polymorphisms of seven base pairs or greater between B73 and Mo17 produce polymorphic markers at a 97% frequency with 49% of the products showing co-dominant fragment length polymorphisms. When the same polymorphisms are used to develop markers for B73 and W22 or Mo17 and W22 mapping populations, 22% and 31% of markers are co-dominant, respectively. There are 38,223 Indel polymorphisms that can be converted to markers providing high-density coverage throughout the maize genome. This strategy significantly increases the efficiency of marker development for fine-mapping in maize.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Marcadores Genéticos
/
Zea mays
/
Polimorfismo de Nucleotídeo Único
/
Mutação INDEL
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article