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Comparative evaluation of the Diagenode multiplex PCR assay on the BD max system versus a routine in-house assay for detection of Bordetella pertussis.
Kenicer, Juliet; Hardie, Alison; Hamilton, Fiona; Gadsby, Naomi; Templeton, Kate.
Afiliação
  • Kenicer J; Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
  • Hardie A; Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
  • Hamilton F; Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
  • Gadsby N; Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
  • Templeton K; Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom Kate.Templeton@luht.scot.nhs.uk.
J Clin Microbiol ; 52(7): 2668-70, 2014 Jul.
Article em En | MEDLINE | ID: mdl-24789178
ABSTRACT
This study looked at 128 nasopharyngeal aspirates (NPA) and 162 throat swabs (TS) tested with the Diagenode multiplex assay on the BD Max system versus our in-house Bordetella pertussis PCR. Sensitivity and specificity were 97.3% and 100% for NPA and 88.3% and 98% for TS, respectively. Of positive NPA, 42.1% were coinfected with respiratory viruses.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bordetella pertussis / Coqueluche / Técnicas de Diagnóstico Molecular / Reação em Cadeia da Polimerase Multiplex Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Adolescent / Adult / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bordetella pertussis / Coqueluche / Técnicas de Diagnóstico Molecular / Reação em Cadeia da Polimerase Multiplex Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Adolescent / Adult / Aged80 / Child / Child, preschool / Female / Humans / Infant / Male / Middle aged Idioma: En Ano de publicação: 2014 Tipo de documento: Article