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Towards error-free profiling of immune repertoires.
Shugay, Mikhail; Britanova, Olga V; Merzlyak, Ekaterina M; Turchaninova, Maria A; Mamedov, Ilgar Z; Tuganbaev, Timur R; Bolotin, Dmitriy A; Staroverov, Dmitry B; Putintseva, Ekaterina V; Plevova, Karla; Linnemann, Carsten; Shagin, Dmitriy; Pospisilova, Sarka; Lukyanov, Sergey; Schumacher, Ton N; Chudakov, Dmitriy M.
Afiliação
  • Shugay M; 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia. [3].
  • Britanova OV; 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2].
  • Merzlyak EM; 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2].
  • Turchaninova MA; 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2].
  • Mamedov IZ; 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
  • Tuganbaev TR; Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
  • Bolotin DA; Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
  • Staroverov DB; Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
  • Putintseva EV; Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
  • Plevova K; Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
  • Linnemann C; Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
  • Shagin D; 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia.
  • Pospisilova S; Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
  • Lukyanov S; 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia.
  • Schumacher TN; Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
  • Chudakov DM; 1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia. [3] Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Nat Methods ; 11(6): 653-5, 2014 Jun.
Article em En | MEDLINE | ID: mdl-24793455
ABSTRACT
Deep profiling of antibody and T cell-receptor repertoires by means of high-throughput sequencing has become an attractive approach for adaptive immunity studies, but its power is substantially compromised by the accumulation of PCR and sequencing errors. Here we report MIGEC (molecular identifier groups-based error correction), a strategy for high-throughput sequencing data analysis. MIGEC allows for nearly absolute error correction while fully preserving the natural diversity of complex immune repertoires.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Projetos de Pesquisa / Receptores de Antígenos de Linfócitos T / Impressões Digitais de DNA / Sequenciamento de Nucleotídeos em Larga Escala Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Projetos de Pesquisa / Receptores de Antígenos de Linfócitos T / Impressões Digitais de DNA / Sequenciamento de Nucleotídeos em Larga Escala Tipo de estudo: Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article