Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling.
Nat Mater
; 13(7): 748-55, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24813421
ABSTRACT
The detection and inactivation of pathogenic strains of bacteria continues to be an important therapeutic goal. Hence, there is a need for materials that can bind selectively to specific microorganisms for diagnostic or anti-infective applications, but that can be formed from simple and inexpensive building blocks. Here, we exploit bacterial redox systems to induce a copper-mediated radical polymerization of synthetic monomers at cell surfaces, generating polymers in situ that bind strongly to the microorganisms that produced them. This 'bacteria-instructed synthesis' can be carried out with a variety of microbial strains, and we show that the polymers produced are self-selective binding agents for the 'instructing' cell types. We further expand on the bacterial redox chemistries to 'click' fluorescent reporters onto polymers directly at the surfaces of a range of clinical isolate strains, allowing rapid, facile and simultaneous binding and visualization of pathogens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
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Pseudomonas aeruginosa
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Cobre
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Escherichia coli
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Polimerização
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article