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Adjustments, extinction, and remains of selenocysteine incorporation machinery in the nematode lineage.
Otero, Lucía; Romanelli-Cedrez, Laura; Turanov, Anton A; Gladyshev, Vadim N; Miranda-Vizuete, Antonio; Salinas, Gustavo.
Afiliação
  • Otero L; Cátedra de Inmunología, Facultad de Química, Instituto de Higiene, Universidad de la República, Montevideo 11600, Uruguay.
  • Romanelli-Cedrez L; Cátedra de Inmunología, Facultad de Química, Instituto de Higiene, Universidad de la República, Montevideo 11600, Uruguay.
  • Turanov AA; Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.
  • Gladyshev VN; Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.
  • Miranda-Vizuete A; Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, 41013 Sevilla, Spain Departamento de Fisiología, Anatomía y Biología Celular, Centro Andaluz de Biología del Desarrollo (CABD-CSIC), Universidad Pablo de Olavide, 41013 Sevilla, Spain.
  • Salinas G; Cátedra de Inmunología, Facultad de Química, Instituto de Higiene, Universidad de la República, Montevideo 11600, Uruguay.
RNA ; 20(7): 1023-34, 2014 Jul.
Article em En | MEDLINE | ID: mdl-24817701
Selenocysteine (Sec) is encoded by an UGA codon with the help of a SECIS element present in selenoprotein mRNAs. SECIS-binding protein (SBP2/SCBP-2) mediates Sec insertion, but the roles of its domains and the impact of its deficiency on Sec insertion are not fully understood. We used Caenorhabditis elegans to examine SBP2 function since it possesses a single selenoprotein, thioredoxin reductase-1 (TRXR-1). All SBP2 described so far have an RNA-binding domain (RBD) and a Sec-incorporation domain (SID). Surprisingly, C. elegans SBP2 lacks SID and consists only of an RBD. An sbp2 deletion mutant strain ablated Sec incorporation demonstrating SBP2 essentiality for Sec incorporation. Further in silico analyses of nematode genomes revealed conservation of SBP2 lacking SID and maintenance of Sec incorporation linked to TRXR-1. Remarkably, parasitic plant nematodes lost the ability to incorporate Sec, but retained SecP43, a gene associated with Sec incorporation. Interestingly, both selenophosphate synthetase (SPS) genes are absent in plant parasitic nematodes, while only Cys-containing SPS2 is present in Sec-incorporating nematodes. Our results indicate that C. elegans and the nematode lineage provide key insights into Sec incorporation and the evolution of Sec utilization trait, selenoproteomes, selenoproteins, and Sec residues. Finally, our study provides evidence of noncanonical translation initiation in C. elegans, not previously known for this well-established animal model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adaptação Biológica / Selenocisteína / Caenorhabditis elegans / Evolução Molecular / Inativação Gênica / Redes e Vias Metabólicas Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adaptação Biológica / Selenocisteína / Caenorhabditis elegans / Evolução Molecular / Inativação Gênica / Redes e Vias Metabólicas Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article