Centromeric histone variant CENP-A represses acetylation-dependent chromatin transcription that is relieved by histone chaperone NPM1.
J Biochem
; 156(4): 221-7, 2014 Oct.
Article
em En
| MEDLINE
| ID: mdl-24839294
ABSTRACT
Mammalian centromeric histone H3 variant, CENP-A, is involved in maintaining the functional integrity and epigenetic inheritance of the centromere. CENP-A causes transcriptional repression of centromeric chromatin through an unknown mechanism. Here, we report that reconstituted CENP-A nucleosomes are amenable to ATP-dependent SWI/SNF-mediated remodelling but are less permissive to acetylation and acetylation-dependent in vitro chromatin transcription. Remarkably, the transcriptional repression of the CENP-A chromatinized template could be relieved by the ectopic addition of histone chaperone, nucleophosmin.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autoantígenos
/
Proteínas Nucleares
/
Cromatina
/
Proteínas Cromossômicas não Histona
/
Histonas
/
Centrômero
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article