Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes.
J Microbiol Methods
; 102: 69-72, 2014 Jul.
Article
em En
| MEDLINE
| ID: mdl-24845469
Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ascaridoidea
/
DNA
/
Biologia Molecular
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article