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Direct, reagentless electrochemical detection of the BIR3 domain of X-linked inhibitor of apoptosis protein using a peptide-based conducting polymer sensor.
Wang, X; Piro, B; Reisberg, S; Anquetin, G; de Rocquigny, H; Jiang, P; Wang, Q; Wu, W; Pham, M-C; Dong, C-Z.
Afiliação
  • Wang X; Lanzhou University, School of Nuclear Science and Technology, Lanzhou 730000, China; University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR CNRS 7086, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
  • Piro B; University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR CNRS 7086, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France. Electronic address: piro@univ-paris-diderot.fr.
  • Reisberg S; University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR CNRS 7086, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
  • Anquetin G; University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR CNRS 7086, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
  • de Rocquigny H; Université de Strasbourg, UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, 67401 Illkirch, France.
  • Jiang P; Lanzhou University, Institute of Cancer Biology and Drug Screening, School of Life Sciences, Lanzhou 730000, China.
  • Wang Q; Lanzhou University, Institute of Cancer Biology and Drug Screening, School of Life Sciences, Lanzhou 730000, China.
  • Wu W; Lanzhou University, School of Nuclear Science and Technology, Lanzhou 730000, China.
  • Pham MC; University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR CNRS 7086, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
  • Dong CZ; University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR CNRS 7086, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; Institute for Interdisciplinary Research, Jianghan University, Wuhan Economic and Technological Development Zone, Wuhan 430056, China. Electronic address: dong@univ-paris-diderot.f
Biosens Bioelectron ; 61: 57-62, 2014 Nov 15.
Article em En | MEDLINE | ID: mdl-24858673
In this work, we report a reagentless electrochemical peptide (AVPFAQKG) sensor to directly detect the BIR3 domain of X-linked inhibitor of apoptosis protein (XIAP-BIR3). The bioreceptor was based on a conducting copolymer film electrosynthesized from juglone and a juglone-peptide conjugate (JP) newly designed. The peptide-protein interactions generated an important increase of steric hindrance at the interface and a current decrease (signal off) of the redox reaction from quinone embedded in the polymer backbone as evidenced by Square Wave Voltammetry. This allowed a specific and sensitive detection of XIAP-BIR3 with a detection limit of 1 nM (13 ng mL(-1)). The peptide-protein complex could be then dissociated by adding the free precursor peptide (AVPFAQKG) into solution, causing a shift-back on the signal, i.e. an increase in the current intensity (signal-on). This "off-on" detection sequence was used in this work as a double verification of the specificity and this approach can be employed as a general way to increase the reliability of the results. In general, the approach described in this work may be inspired to develop other direct and reagentless electrochemical protein assays with high specificity and sensitivity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Técnicas Biossensoriais / Naftoquinonas / Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X / Técnicas Eletroquímicas Tipo de estudo: Diagnostic_studies / Evaluation_studies / Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligopeptídeos / Técnicas Biossensoriais / Naftoquinonas / Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X / Técnicas Eletroquímicas Tipo de estudo: Diagnostic_studies / Evaluation_studies / Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article