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Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing.
Nielsen, Karen L; Godfrey, Paul A; Stegger, Marc; Andersen, Paal S; Feldgarden, Michael; Frimodt-Møller, Niels.
Afiliação
  • Nielsen KL; Department of Clinical Microbiology, Hvidovre Hospital, 2640 Hvidovre, Denmark; Department of Microbiology and Infection Control, Statens Serum Institut, 2300 Copenhagen S, Denmark. Electronic address: KTE@ssi.dk.
  • Godfrey PA; Genome Sequencing and Analysis Program, Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Stegger M; Department of Microbiology and Infection Control, Statens Serum Institut, 2300 Copenhagen S, Denmark.
  • Andersen PS; Department of Microbiology and Infection Control, Statens Serum Institut, 2300 Copenhagen S, Denmark.
  • Feldgarden M; Genome Sequencing and Analysis Program, Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Frimodt-Møller N; Department of Clinical Microbiology, Hvidovre Hospital, 2640 Hvidovre, Denmark.
J Microbiol Methods ; 103: 101-3, 2014 Aug.
Article em En | MEDLINE | ID: mdl-24912108
Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnica de Amplificação ao Acaso de DNA Polimórfico / Escherichia coli Tipo de estudo: Clinical_trials / Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnica de Amplificação ao Acaso de DNA Polimórfico / Escherichia coli Tipo de estudo: Clinical_trials / Prognostic_studies Idioma: En Ano de publicação: 2014 Tipo de documento: Article