Quantitative pH assessment of small-volume samples using a universal pH indicator.
Anal Biochem
; 462: 29-31, 2014 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-24929087
ABSTRACT
We developed a hue-based pH determination method to analyze digital images of samples in a 384-well plate after the addition of a universal pH indicator. The standard error of calibration for 69 pH standards was 0.078 pH units, and no sample gave an error greater than 0.23 units. We then used in-solution isoelectric focusing to determine the isoelectric point of Wnt3A protein in conditioned medium and after purification and applied the described method to assess the pH of these small-volume samples. End users may access our standard to assay the pH of their own samples with no additional calibration.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Focalização Isoelétrica
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article