Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system.
Mol Cells
; 37(6): 497-502, 2014 Jun.
Article
em En
| MEDLINE
| ID: mdl-24938227
ABSTRACT
Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
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Técnicas Analíticas Microfluídicas
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Células-Tronco Embrionárias
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Neurônios
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article