A conserved mitochondrial ATP-binding cassette transporter exports glutathione polysulfide for cytosolic metal cofactor assembly.
J Biol Chem
; 289(34): 23264-74, 2014 Aug 22.
Article
em En
| MEDLINE
| ID: mdl-25006243
ABSTRACT
An ATP-binding cassette transporter located in the inner mitochondrial membrane is involved in iron-sulfur cluster and molybdenum cofactor assembly in the cytosol, but the transported substrate is unknown. ATM3 (ABCB25) from Arabidopsis thaliana and its functional orthologue Atm1 from Saccharomyces cerevisiae were expressed in Lactococcus lactis and studied in inside-out membrane vesicles and in purified form. Both proteins selectively transported glutathione disulfide (GSSG) but not reduced glutathione in agreement with a 3-fold stimulation of ATPase activity by GSSG. By contrast, Fe(2+) alone or in combination with glutathione did not stimulate ATPase activity. Arabidopsis atm3 mutants were hypersensitive to an inhibitor of glutathione biosynthesis and accumulated GSSG in the mitochondria. The growth phenotype of atm3-1 was strongly enhanced by depletion of the mitochondrion-localized, GSH-dependent persulfide oxygenase ETHE1, suggesting that the physiological substrate of ATM3 contains persulfide in addition to glutathione. Consistent with this idea, a transportomics approach using mass spectrometry showed that glutathione trisulfide (GS-S-SG) was transported by Atm1. We propose that mitochondria export glutathione polysulfide, containing glutathione and persulfide, for iron-sulfur cluster assembly in the cytosol.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfetos
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Sequência Conservada
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Transportadores de Cassetes de Ligação de ATP
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Citosol
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Proteínas de Saccharomyces cerevisiae
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Proteínas Mitocondriais
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Proteínas de Arabidopsis
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Glutationa
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Metais
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article