RNA interference-mediated knockdown of brain-derived neurotrophic factor (BDNF) promotes cell cycle arrest and apoptosis in B-cell lymphoma cells.
Neoplasma
; 61(5): 523-32, 2014.
Article
em En
| MEDLINE
| ID: mdl-25030435
ABSTRACT
Brain-derived neurotrophic factor (BDNF) is aâ¯member of the neurotrophin superfamily that has been reported to be involved in aâ¯number of neurological and psychological situations. Recently, high expression level of BDNF is observed in diverse human malignancies, delineating aâ¯role of BDNF in tumorigenesis. Nevertheless, its effect on B-cell lymphoma remains unclear. In this study, RNA interference technology mediated by short hairpin RNA (shRNA) was performed to inhibit endogenous BDNF expression in B-cell lymphoma cells. Results showed that knockdown of BDNF reduced cell growth and proliferation of Raji and Ramos cells. Furthermore, down-regulation of BDNF induced aâ¯cell cycle arrest at G0/G1 phase in Raji cells, and consequently led to cell apoptosis in vitro. Meanwhile, down-regulation of Bcl-2 and up-regulation of Bax, activated caspase-3 and caspase-9 and cleaved poly (ADP-ribose) polymerase (PARP) were observed in Raji cells when endogenous BDNF was inhibited. Besides, we also found that suppression of BDNF in Raji cells increased their sensitivity to chemotherapeutic drug, 5-Fluorouracil (5-FU). Our research provides aâ¯promising therapeutic strategy for human B-cell lymphoma by targeting BDNF.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfoma de Células B
/
Apoptose
/
Fator Neurotrófico Derivado do Encéfalo
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Interferência de RNA
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article