Screening and selection of synthetic peptides for a novel and optimized endotoxin detection method.
J Biotechnol
; 186: 162-8, 2014 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-25034430
ABSTRACT
The current validated endotoxin detection methods, in spite of being highly sensitive, present several drawbacks in terms of reproducibility, handling and cost. Therefore novel approaches are being carried out in the scientific community to overcome these difficulties. Remarkable efforts are focused on the development of endotoxin-specific biosensors. The key feature of these solutions relies on the proper definition of the capture protocol, especially of the bio-receptor or ligand. The aim of the presented work is the screening and selection of a synthetic peptide specifically designed for LPS detection, as well as the optimization of a procedure for its immobilization onto gold substrates for further application to biosensors.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Técnicas Biossensoriais
/
Lipopolissacarídeos
/
Proteínas Imobilizadas
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article