Synthesis of biotinylated keratan sulfate repeating disaccharides.

ABSTRACT

We synthesized four types of keratan and keratan sulfate repeating disaccharides containing non-sulfate, Galß1-4GlcNAcß, and three types of sulfates, Gal6Sß1-4GlcNAcß, Galß1-4GlcNAc6Sß, and Gal6Sß1-4GlcNAc6Sß in an efficient and stereo-controlled manner. These disaccharides were conjugated with biotin via a hydrophilic linker at the reducing terminal.