A tailor-made chimeric thiamine diphosphate dependent enzyme for the direct asymmetric synthesis of (S)-benzoins.
Angew Chem Int Ed Engl
; 53(35): 9376-9, 2014 Aug 25.
Article
em En
| MEDLINE
| ID: mdl-25044968
ABSTRACT
Thiamine diphosphate dependent enzymes are well known for catalyzing the asymmetric synthesis of chiral α-hydroxy ketones from simple prochiral substrates. The steric and chemical properties of the enzyme active site define the product spectrum. Enzymes catalyzing the carboligation of aromatic aldehydes to (S)-benzoins have not so far been identified. We were able to close this gap by constructing a chimeric enzyme, which catalyzes the synthesis of various (S)-benzoins with excellent enantiomeric excess (>99%) and very good conversion.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tiamina Pirofosfato
/
Piruvato Descarboxilase
/
Benzoína
/
Aldeído Liases
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article