Monitoring Notch activation in cultured mammalian cells: transcriptional reporter assays.
Methods Mol Biol
; 1187: 143-54, 2014.
Article
em En
| MEDLINE
| ID: mdl-25053487
Upon ligand binding, Notch activation and cleavage culminates in the expression of its target genes. Hence, the use of Notch-responsive promoters to drive reporter gene expression provides a flexible and robust approach for monitoring signaling activity. In this chapter, we present an overview of Notch transcriptional reporter assays and discuss different methods for ligand-independent and ligand-dependent activation of Notch in mammalian cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Ativação Transcricional
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Receptores Notch
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article