Single-cell RNA-seq: advances and future challenges.
Nucleic Acids Res
; 42(14): 8845-60, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-25053837
Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Single-cell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. Different single-cell RNA-seq protocols have been introduced and are reviewed here-each one with its own strengths and current limitations. We further provide an overview of the biological questions single-cell RNA-seq has been used to address, the major findings obtained from such studies, and current challenges and expected future developments in this booming field.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de RNA
/
Perfilação da Expressão Gênica
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Análise de Célula Única
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Sequenciamento de Nucleotídeos em Larga Escala
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article