The in vitro effects of sodium salicylate on von Willebrand factor and C-reactive protein production by endothelial cells.
Inflammopharmacology
; 22(6): 367-72, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25055995
ABSTRACT
OBJECTIVE:
The role of inflammatory and endothelial dysfunction markers in the atherogenic process has been well recognized. The data have made both C-reactive protein (CRP) and von Willebrand factor (vWF) promising targets for the cardiovascular disease research and drug development. Inhibition of CRP and vWF synthesis, therefore, might be a potential therapeutic strategy.METHODS:
The effect of sodium salicylate on vWF production by human umbilical vein endothelial cells (HUVECs) using enzyme-linked immunosorbent Assays (ELISA) and real-time PCR was examined. In addition, small interfering RNA (siRNA) against NF-κB was used to investigate the existence of a role for this signaling pathway.RESULTS:
Our findings demonstrated that sodium salicylate decreased vWF, but not CRP production at both mRNA and protein levels significantly and this might not occur via nuclear transcription factor (NF-κB) inhibition.CONCLUSION:
Our results indicated a further rationalization of the effects of sodium salicylate on atherothrombotic events by attenuation of vWF production.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína C-Reativa
/
Fator de von Willebrand
/
Salicilato de Sódio
/
Células Endoteliais da Veia Umbilical Humana
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article